Biological activities and determination of the mode of action of Tunisian Globularia alypum and Cistus monspeliensis ethanolic extracts.
- Resource Type
- Academic Journal
- Authors
- Nefzi K; National Research Institute of Rural Engineering, Water and Forests (INRGREF), LR11INRGREF0 Laboratory of Management and Valorization of Forest Resources, Carthage University, Ariana, Tunisia.; Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, Belvédère, Tunis.; Charfi K; Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, Belvédère, Tunis.; Maaroufi A; Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, Belvédère, Tunis.; Hosni K; Laboratoire des substances naturelles, Institut National de Reherche et d'Analyse Physico-chimiques (IRAP), Sidi Thabet, Tunisia.; Msaada K; Laboratory of Aromatic and Medicinal Plants (LPAM), Biotechnology Center in Borj Cedria Technopole, Hammam-lif, Tunisia.; Baraket M; National Research Institute of Rural Engineering, Water and Forests (INRGREF), LR11INRGREF0 Laboratory of Management and Valorization of Forest Resources, Carthage University, Ariana, Tunisia.; Nasr Z; National Research Institute of Rural Engineering, Water and Forests (INRGREF), LR11INRGREF0 Laboratory of Management and Valorization of Forest Resources, Carthage University, Ariana, Tunisia.
- Source
- Publisher: Informa Healthcare Country of Publication: England NLM ID: 9106628 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1369-1619 (Electronic) Linking ISSN: 09603123 NLM ISO Abbreviation: Int J Environ Health Res Subsets: MEDLINE
- Subject
- Language
- English
This study was designed to evaluate the antioxidant and antimicrobial activity of ethanolic extracts (EEs) of Cistus monspeliensis and Globularia alypum . C. monspeliensis showed the highest values of phenolic compounds. Further, it was shown that EE of C. monspeliensis displayed the highest DPPH (IC50 = 8.3 ± 1.08 mg/mL). The chemical profiles demonstrated a total of 12 and 13 phenolic compounds for C. monspeliensis and G. alypum , respectively. EEs of both plants possessed broad-spectrum antimicrobial activity when tested against Escherichia coli and Staphylococcus aureus . Treatment of studied strains with these extracts at their MICs reduced considerably the bacterial viability. The extracts did not induce total bacterial lysis, as determined by the measurement of optical density at 620 nm. Strains treated with EEs at Minimum Inhibitory Concentrations showed significant loss of tolerance to NaCl. Our results contribute to understanding the antibacterial mechanism of ethanolic extracts of the studied medicinal plants.