The difficulty in producing multi-carbon and thus high-value chemicals from CO 2 is one of the key challenges of microbial electrosynthesis (MES) and other CO 2 utilization technologies. Here, we demonstrate a two-stage bioproduction approach to produce terpenoids (>C 20 ) and yeast biomass from CO 2 by linking MES and yeast cultivation approaches. In the first stage, CO 2 (C 1 ) is converted to acetate (C 2 ) using Clostridium ljungdahlii via MES. The acetate is then directly used as the feedstock to produce sclareol (C 20 ), β-carotene (C 40 ), and yeast biomass using Saccharomyces cerevisiae in the second stage. With the unpurified acetate-containing (1.5 g/L) spent medium from MES reactors, S. cerevisiae produced 0.32 ± 0.04 mg/L β-carotene, 2.54 ± 0.91 mg/L sclareol, and 369.66 ± 41.67 mg/L biomass. The primary economic analysis suggests that sclareol and biomass production is feasible using recombinant S. cerevisiae and non-recombinant S. cerevisiae, respectively, directly from unpurified acetate-containing spent medium of MES.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Sunil Anil Patil, Anand Kumar Bachhawat, Ravineet Yadav, Banani Chattopadhyay has patent #A TWO-STAGE PROCESS FOR PRODUCING TERPENES AND YEAST BIOMASS FROM CO2 AND SYSTEM THEREOF pending to Intellectal Property India.
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