Structural and physicochemical characterisation and antioxidant activity of an α-D-glucan produced by sourdough isolate Weissella cibaria MED17.
- Resource Type
- Article
- Authors
- Aburas, Hani; İspirli, Hümeyra; Taylan, Osman; Yilmaz, Mustafa Tahsin; Dertli, Enes
- Source
- International Journal of Biological Macromolecules. Oct2020, Vol. 161, p648-655. 8p.
- Subject
- *BETA-glucans
*DEXTRAN
*ANTIOXIDANTS
*THERMAL stability
- Language
- ISSN
- 0141-8130
An exopolysaccharide (EPS) producer slimy-mucoid type colony was isolated from sourdough and identified as Weissella cibaria MED17. The 1H and 13C NMR spectra of EPS MED17 demonstrated that this EPS was a dextran type glucan ((1 → 6)-linked α-D-glucose core structure) containing (1 → 3)-linked α-D-glucose branches and proportion of (1 → 6)-linked α-D-glucose units to (1 → 3)-linked α-D-glucose units was 94.3:5.7%. The FTIR analysis also confirmed the (1 → 6)-linked α-D-glucose linkage. A high level of thermal stability was observed for glucan MED17 as no degradation up to 300 °C was observed by TGA and DSC analysis. The XRD analysis of glucan MED17 showed its semi- crystalline nature and its compact sheet-like morphology was observed by SEM analysis. Finally, antioxidant characteristics of glucan MED17 were determined by ABTS and DPPH radical scavenging activity tests that revealed a moderate antioxidant activity of glucan MED17. These findings show potential techno-functional characteristics of glucan MED17. • Weissella cibaria MED17 as a glucan producer was isolated from sourdough. • Proportion of α(1 → 6) to α(1 → 3) units was 94.3:5.7% in glucan MED17. • No degradation up to 300 °C was observed by TGA and DSC analysis. • Glucan MED17 had semi-crystalline nature and compact sheet-like morphology. • Glucan MED17 showed moderate antioxidant activity. [ABSTRACT FROM AUTHOR]