[Objective] to explore and evaluate the safety of Jin Weng Zhi Li Extract on chickens. [Methods] 60 healthy chickens (3 weeks old) from Rongchang were grouped into four groups, i. e., blank group , one time the recommended dose group (0.4 g/kg), three times the recommended dose group (1.2g/kg), five times the recommended dose group (2.0 g/kg). Chickens in blank group were fed with norma saline by gastric infusion (0.5 mL per chicken) , and chickens were fed with Jin Weng Zhi Li extract solution by gastric infusion, 2 times a day, 14 consecutive days. During the experiment, the clinical manifestation of chickens were observed, the three major physiological indexes (breathing, pulse, body temperature), daily weight gain, blood routine examination and blood biochemical indexes were determined. The chickens used were dissected at the end of the experiment. Their internal organs were observed to determine if there're macroscopic lesions, and the organs were made into paraffin sections. Then histological observation of the paraffin sections was performed. [Results] The three major physiological indexes, blood routine examination and blood biochemical indexes were all within normal range and of no significant difference (P > 0.05); and no pathological lesions were found by observing the tissue section. [Conclusions] The experimental results indicated that the effect of Jin Weng Zhi Li Extract on the clinical manifestation, haematology and histology was within normal range, and it can be used clinically, and the dose of 1 time the recommended dose and 5 times the recommended dose was within the safe dose range. [ABSTRACT FROM AUTHOR]