The Additional 15 nt of 5′ UTR in a Novel Recombinant Isolate of Chilli Veinal Mottle Virus in Solanum nigrum L. Is Crucial for Infection.
- Resource Type
- Article
- Authors
- Wan, Qionglian; Zheng, Kaiyue; Wu, Jian; Bu, Shan; Jiao, Mengting; Zhou, Huijie; Lu, Yuwen; Zheng, Hongying; Wu, Guanwei; Rao, Shaofei; Chen, Hairu; Yan, Fei; Peng, Jiejun
- Source
- Viruses (1999-4915). Jul2023, Vol. 15 Issue 7, p1428. 12p.
- Subject
- *SOLANUM nigrum
*CAPSICUM annuum
*VIRUS cloning
*NICOTIANA benthamiana
*POTYVIRUSES
*GENETIC variation
- Language
- ISSN
- 1999-4915
An isolate of chilli veinal mottle virus (ChiVMV; genus Potyvirus) of Solanum nigrum L. from southwest China (ChiVMV-YunN/Yuxi) was identified and sequenced (GenBank: OP404087). Comparison with other ChiVMV isolates and recombination analyses suggested a recombinant origin. The most significant recombination event among all 21 complete ChiVMV isolates was an ending breakpoint at 1408–1488 for ChiVMV-YunN/Yuxi with ChiVMV-TaiW and ChiVMV-YunN/Ca operating as the respective major and minor parents. Interestingly, the 5′ UTR of ChiVMV-YunN/Yuxi is 15 nucleotides ('AAAAATAAAACAACC') longer than other reported isolates. A full-length clone of ChiVMV-YunN/Yuxi was constructed and was shown to be infectious in Nicotiana benthamiana. The additional 15 nt of 5′ UTR in ChiVMV-YunN/Yuxi was stable when transmitted through three generations. Experiments with modified clones showed that the additional 15 nt are essential for infection by this isolate. [ABSTRACT FROM AUTHOR]