Data derived from forest maps and ground surveys was used to study habitat preferences and sexual segregation in two populations of northern white-tailed deer (Odocoileus virginianus) living at low density in southeastern Quebec. For ungulates living in seasonal environments, the habitat preferences of the sexes should diverge the most during late spring and summer because this period coincides with the time when needs associated with lifetime reproductive success are the most divergent. It is important to estimate habitat use as the area composed by each habitat type within individual home ranges because a preliminary analysis revealed that area measurements yielded a similar distribution of habitat use as telemetry locations (P 0.09 and 0.14 for Pohénégamook(PWA) and Lac Témiscouata (LTWA) populations. Habitat availability was relatively similar for the two deer populations except for agricultural lands, which covered proportionally more area in the LTWA than in the PWA range, and clearcuts, which were more common in the PWA summer range.