In Vivo Versus In Vitro Airway Surface Liquid Nicotine Levels Following Cigarette Smoke Exposure.
- Resource Type
- Article
- Authors
- Clunes, Lucy A.; Bridges, Arlene; Alexis, Neil; Tarran, Robert
- Source
- Journal of Analytical Toxicology. Apr2008, Vol. 32 Issue 3, p201-207. 7p. 2 Diagrams, 2 Graphs.
- Subject
- *TOBACCO smoke
*CIGARETTE smoke
*PHYSIOLOGICAL effects of tobacco
*NICOTINE metabolism
*NICOTINE addiction
*LABORATORY techniques
*COTININE
*CIGARETTE industry
*SPUTUM microbiology
- Language
- ISSN
- 0146-4760
The article discusses the system for exhibiting epithelial cells cultivated at an air-liquid port to whole cigarette smoke (WCS). According to the author the use of WSC in vitro was more applicable to what is seen in vivo than methods of cigarette smoke application that only use a small fraction of WCS. A comparison of nicotine and cotinine absorptions in the airway surface liquid of human primary bronchial epithelial cultures was also discussed. It is suggested that nicotine levels are not different in induced sputum when measured by mass spectrometry.