Traumatic events can have a detrimental impact on individuals' health and well-being. Ensuring trauma-informed care (TIC) in key community sectors is an important step in addressing trauma. We conducted an organizational assessment to identify the strengths and needs of organizations in implementing TIC in three sectors located in a Midwestern mid-size city: first responder organizations, health care institutions, and a child welfare agency. Using an explanatory sequential mixed-methods design, middle-level managers (n = 118) from the three sectors participated in online surveys and follow-up focus groups (n = 25). We assessed participants' self-reported experiences across the following organizational domains: staff training (knowledge and skills), leadership commitment, organizational policies, and staff supervision. Sectors differed in their organizational strengths and needs related to the TIC. The first responder organizations reported well-established policies and de-briefing programs, with a greater need for trauma-informed training and practical support. Healthcare institutions reported high levels of training in patient screening and referrals, but expressed less effective communication within the organization and unstructured resources for TIC services. The child welfare sector showed the highest level of understanding about TIC through their strong internal training programs, but challenges exist in applying the training to daily practice and dealing with vicarious trauma for staff. We discuss the implications of these findings and suggest sector-specific organizational strategies. [ABSTRACT FROM AUTHOR]