A novel nano-molecular imprinting polymer-based electrochemical sensor for follicle-stimulating hormone (FSH) in polycystic ovary syndrome (PCOS) has been developed. The present methods available for PCOS detection are associated with several disadvantages such as they are costly, time-consuming process, and low sensitivity. This proposed biosensor can help to overcome these conditions while providing a dynamic detection range, a lower limit of detection, higher sensitivity, rapid detection, and low-cost detection. As this biosensor is based on the molecular imprinting approach it is highly specific and stable at a higher temperature. To our knowledge, no such biosensor is developed for the diagnosis of PCOS. Highly selective and specific recognition of the follicle-stimulating hormone-molecular imprinting polymer (FSH-MIP) film onto the nanomaterial (NiCo 2 O 4 /rGO) modified indium tin oxide (ITO) electrode was done by electropolymerization method. Characterization and behavior of the electrode surface fabricated were investigated by using various surface and electrochemical measurements. Under optimal conditions, the detection limit and linear working range obtained were 0.1 pM and 0.1 pM to 1 µM, respectively. This fabricated biosensor is simple, rapid, and sensitive for the electrochemical detection of FSH based on the MIP technique. • A novel FSH-MIP-based nano biosensor for PCOS detection. • The linear range obtained was 0.1pM to 1 µM. • The detection limit was calculated as 0.1pM. • This fabricated biosensor is simple, rapid, and sensitive. [ABSTRACT FROM AUTHOR]