Nutrient overloading resulting from digestate (effluent of anaerobic digestion process) application has become a major bottleneck for the development of the biogas industry and raised environmental concerns in regions with intensive animal husbandry. Due to this, it is imperative to find low cost and effective alternative to export nutrient from digestate. Among the numerous applications, indigenous microflora has recently been utilized successfully as a biofloc technology in aquatic systems for controlling ammonia and subsequent reduction of feeding cost. Accordingly, performance of the indigenous microflora in undiluted liquid digestate of chicken manure was evaluated in this study to recover nutrients and produce high-value biomass under aerobic heterotrophic mode in batch shaking experiments. The results showed that 68% of phosphate was recovered and 97% of total nitrogen was removed from the liquid digestate. Additionally, >6 g L−1 of dry biomass was simultaneously produced and featured with up to 65% crude protein without pathogens, 10.9% lipids, 10.7% ash and 19.6 MJ kg−1 gross energy. Therefore, the produced biomass could be used either as an alternative sustainable source for animal or fish feeding or as a substrate for energy applications. Unlabelled Image • Heterotrophic indigenous microflora grew in undiluted liquid digestate successfully. • Phosphate & total nitrogen recovery were 68% & 19%, total nitrogen removal was 97%. • >6 g L−1 of dry biomass was simultaneously produced without pathogens. • Biomass features with 65% crude protein, 10.9% lipids and 19.6 MJ kg−1 gross energy • Integrated production of microbial biomass and ammonium salts is recommended. [ABSTRACT FROM AUTHOR]