Up-Streaming Process for Glucose Oxidase by Thermophilic Pénicillium sp. in Shake Flask.
- Resource Type
- Article
- Authors
- Javed, Muhammad Mohsin; Shabbir, Aroosh; Zahoor, Sana; Ul-Haq, Ikram
- Source
- Walailak Journal of Science & Technology. Jun2012, Vol. 9 Issue 2, p127-133. 7p.
- Subject
- *GLUCOSE oxidase
*THERMOPHILIC microorganisms
*FERMENTATION
*SOIL microbiology
*EGG incubation
- Language
- ISSN
- 1686-3933
The present study is concerned with the production of glucose oxidase (GOD) from thermophilic Pénicillium sp. in a 250 mL shake flask. Fourteen different strains of thermophilic Pénicillium sp. were isolated from the soil and were screened for glucose oxidase production. IIBP-13 strain gave maximum extra-cellular glucose oxidase production as compared to other isolates. The effect of submerged fermentation in shaking and static conditions, different carbon sources and incubation period on the production of extra-cellular glucose oxidase was studied. A maximum yield (0.325 U/mL) of extra-cellular glucose oxidase was obtained after 72 h of incubation at 45 °C using submerged fermentation under shaking utilizing sucrose as a carbon source. [ABSTRACT FROM AUTHOR]