In this investigation, a series of novel random bio-based thiophene-aromatic copolyesters, including thiophene and phenyl units, were successfully prepared from dimethyl 2,5-thiophenedicarboxylate, dimethyl 2,5-dimethoxyterephthalate, and the aliphatic diols ethylene glycol, 1,3-propanediol, 1,4-butanediol, 1,6-hexanediol and 1,8-octanediol, via twostep melt polymerization as a facile and green semi-continuous process. Techniques used to monitor the polymerization process included Fourier transform infrared (FTIR) and proton nuclear magnetic resonance spectroscopy (¹H NMR), gel permeation chromatography (GPC), differential scanning calorimetry, thermogravimetric analysis, and dynamic mechanical analysis. The copolymers had tunable Tg values ranging from 47-120°C, while their 5% decomposition temperature (Td, 5%) under N2 varied from 40-370 °C. GPC analysis showed that the polyesters had weight-average molecular weights (Mw) of 41 400-48 500 g/mol and polydispersity indices of 1.47-2.24. A study of structure-property relations showed that the properties of these copolymers can be tailored by varying their composition. All the copolyesters exhibited high tensile strength (45--80 MPa) and toughness values (elongation at break of 200-540%). After 28 weeks of incubation in humid soil, weight losses of up to 7.2% were observed. Considering their good mechanical properties, thermal stability and biodegradability, these renewable sulfur-containing copolyesters have great potential to replace petroleum-based commercial poly(ethylene terephthalate) in the food packaging industry, which is helpful to implement carbon neutrality and sustainable development in the polymer industry. [ABSTRACT FROM AUTHOR]