Estimation of soluble β-glucan content of yeast cell wall by the sensitivity to Glucanex® 200G treatment
- Resource Type
- Article
- Authors
- Kim, Kwang Suk; Chang, Jung Eun; Yun, Hyun Shik
- Source
- Enzyme & Microbial Technology. Dec2004, Vol. 35 Issue 6/7, p672-677. 6p.
- Subject
- *GLUCANS
*YEAST
*CELLS
*POLYSACCHARIDES
- Language
- ISSN
- 0141-0229
The soluble β-glucan contents in the cell wall of yeasts were estimated by treating cells with Glucanex® 200G that contained mainly β1,3-glucanase and some β1,6-glucanase. The sensitivity of cell walls of 11 yeasts to various concentrations of β-glucanase was compared. The yeasts that are resistant to β-glucanase treatment are expected to contain higher β-glucan content and those that are sensitive to the β-glucanase treatment are expected to contain lower β-glucan content. Two yeast strains were selected for further study by comparing the sensitivity of cell wall to β-glucanase; Candida bombicola and Candida albicans. Candida bombicola was more resistant and C. albicans was more sensitive to the Glucanex® 200G treatment. The results of enzyme sensitivity tests were verified by quantification of soluble β-glucan content purified from the yeasts. Much larger amount of soluble β-glucan was obtained from the cell walls of C. bombicola (0.08gg-1 dried cell) than C. albicans (0.025gg-1 dried cell). [Copyright &y& Elsevier]