This article discusses the development of glufosinate-resistant rice germplasm through precision editing of the GLR1 gene. Glufosinate is a widely used herbicide, but there have been few reports on rice germplasm that is resistant to it. The researchers obtained glufosinate-resistant mutants from a rice cultivar and conducted experiments to validate their resistance. They found that the glufosinate-resistant mutant exhibited improved physiological indices compared to the wild type, such as survival rate, chlorophyll content, and plant length. The researchers also investigated the molecular basis of glufosinate resistance and identified the GLR1 gene as a key regulator. They found that the GLR1 gene encodes an Auxin response factor and suppresses the expression of downstream genes involved in ammonia and reactive oxygen species (ROS) metabolism. The glufosinate-resistant trait in the mutant was found to be the result of a recessive mutation in the GLR1 gene. The researchers further explored the potential of modifying the GLR1 gene to confer glufosinate resistance without yield penalty in rice. They used CRISPR-Cas9 gene editing to modify different domains of the GLR1 gene and found that retention of certain domains can trade-off between resistance and grain yield. The researchers also discovered that the GLR1 mutation provides tolerance to salt stress and glyphosate herbicide. They suggest that engineering the GLR1 gene has the potential to improve tolerance to abiotic stress in [Extracted from the article]