Gene Mapping and Identification of a Missense Mutation in One Copy of VRN-A1 Affects Heading Date Variation in Wheat.
- Resource Type
- Article
- Authors
- Xue, Qianwen; Xiong, Hongchun; Zhou, Chunyun; Guo, Huijun; Zhao, Linshu; Xie, Yongdun; Gu, Jiayu; Zhao, Shirong; Ding, Yuping; Xu, Le; Liu, Luxiang
- Source
- International Journal of Molecular Sciences. Mar2023, Vol. 24 Issue 5, p5008. 12p.
- Subject
- *GENE mapping
*MISSENSE mutation
*LOCUS (Genetics)
*WHEAT breeding
*GENETIC regulation
*WHEAT
*MOLECULAR cloning
WHEAT genetics
- Language
- ISSN
- 1661-6596
Heading date (HD) is an important trait for wide adaptability and yield stability in wheat. The Vernalization 1 (VRN1) gene is a key regulatory factor controlling HD in wheat. The identification of allelic variations in VRN1 is crucial for wheat improvement as climate change becomes more of a threat to agriculture. In this study, we identified an EMS-induced late-heading wheat mutant je0155 and crossed it with wide-type (WT) Jing411 to construct an F2 population of 344 individuals. Through Bulk Segregant Analysis (BSA) of early and late-heading plants, we identified a Quantitative Trait Locus (QTL) for HD on chromosome 5A. Further genetic linkage analysis limited the QTL to a physical region of 0.8 Mb. Cloning and sequencing revealed three copies of VRN-A1 in the WT and mutant lines; one copy contained a missense mutation of C changed to T in exon 4 and another copy contained a mutation in intron 5. Genotype and phenotype analysis of the segregation population validated that the mutations in VRN-A1 contributed to the late HD phenotype in the mutant. Expression analysis of C- or T-type alleles in exon 4 of the WT and mutant lines indicated that this mutation led to lower expression of VRN-A1, which resulted in the late-heading of je0155. This study provides valuable information for the genetic regulation of HD and many important resources for HD refinement in wheat breeding programs. [ABSTRACT FROM AUTHOR]