Human recombinant phosphodiesterase 4B2B binds (R)-rolipram at a single site with two affinities.
- Resource Type
- Article
- Authors
- Rocque, Warren J.; Luther, Michael A.; Tian, Gaochao; Wiseman, Jeffrey S.; Holmes, William D.; Zajac-Thompson, Irene; Willard, Derril H.; Patel, Indravaden R.; Wisely, G. Bruce; Clay, William C.; Kadwell, Sue H.; Hoffman, Christine R.
- Source
- Biochemistry. 11/18/97, Vol. 36 Issue 46, p14250. 12p. 8 Diagrams, 3 Charts, 14 Graphs.
- Subject
- *PHOSPHODIESTERASES
- Language
- ISSN
- 0006-2960
Studies interactions between human recombinant phosphodiesterase (HSPDE) 4B2B and (R)-rolipram to determine the binding system affinity. Generation and purification of HSPDE4B2B; PDE activity assays; Time-dependent inhibition of PDE by rolipram; Inhibition kinetics; Job-filter binding assays; Equilibrium dialysis.