Proton-coupled electron hopping in Ru-modified P. aeruginosa azurin.
- Resource Type
- Article
- Authors
- Warren, Jeffrey; Shafaat, Oliver; Winkler, Jay; Gray, Harry
- Source
- Journal of Biological Inorganic Chemistry (JBIC). Mar2016, Vol. 21 Issue 1, p113-119. 7p.
- Subject
- *PROTONS
*COPPER proteins
*RUTHENIUM
*PSEUDOMONAS
*HOPPING conduction
*CHARGE exchange
- Language
- ISSN
- 0949-8257
We constructed two artificial multiple-step electron transfer (hopping) systems based on Pseudomonas aeruginosa azurin where a tyrosine (YOH) is situated between Ru(2,2′-bipyridine)(imidazole)(histidine) and the native copper site: RuH107YOH109 and RuH124-YOH122. We investigated the rates of Cu oxidation by flash-quench generated Ru over a range of conditions that probed the role of proton-coupled oxidation/reduction of YOH in the reaction. Rates of Cu oxidation were enhanced over single-step electron transfer by factors between 3 and 80, depending on specific scaffold and buffer conditions. [ABSTRACT FROM AUTHOR]