A robust, sensitive assay for genomic uracil determination by LC/MS/MS reveals lower levels than previously reported.
- Resource Type
- Article
- Authors
- Galashevskaya, Anastasia; Sarno, Antonio; Vågbø, Cathrine B.; Aas, Per A.; Hagen, Lars; Slupphaug, Geir; Krokan, Hans E.
- Source
- DNA Repair. Sep2013, Vol. 12 Issue 9, p699-706. 8p.
- Subject
- *BIOLOGICAL assay
*URACIL
*NUCLEOTIDES
*CYTOSINE deaminase
*DNA repair
- Language
- ISSN
- 1568-7864
Highlights: [•] We analyzed error sources in genomic uracil assays. [•] We present a method that ameliorates possible technical shortcomings. [•] Nucleotides co-eluted in DNA isolation step, causing overestimation. [•] In vitro cytosine deamination to uracil caused overestimation. [•] We report 400–600 uracils per genome in repair-proficient cells. [ABSTRACT FROM AUTHOR]