Among many non-edibles oil trees only few such as linseed, castor, Karanja, neem, rubber, Jatropha and cashew have caught the interest of researchers for biodiesel production others not in use. In this work, for the first time Daturametel is considered as a source for the production of biodiesel. The plant is native to Asia and Africa. This underutilized plant is grown on roadside as ornamental plant and occurs throughout India. It is called as Vellaiummathai in Tamilnadu, India. And it belongs to the Solanaceae family in the Plantae Kingdom. This plant tolerates all kinds of soil types and it can grow best in rich, light sandy soil. From the survey of literatures, it is known for its antibacterial activity and antifungal activity against phyto pathogens also meant to serve as an insecticide but not for biodiesel production. It is observed with the solvent amount of 150ml and 25 grams of Datura metel seed with a particle size of 0.21 mm, the yield of oil from soxhlet apparatus is found to be around 30.8%. Further it is observed that the yield of Biodiesel from transesterification method with process parameters optimized for maximum yield is found to be around 36.25 %. Based on the above experimental analysis, it can be inferred that Datura metel seed is a viable low cost biodiesel which can be explored in the future. [ABSTRACT FROM AUTHOR]