Sensitive detection of the c- KIT c. 1430G>T mutation by mutant-specific polymerase chain reaction in feline mast cell tumours.
- Resource Type
- Article
- Authors
- Takanosu, M.; Sato, M.; Kagawa, Y.
- Source
- Veterinary & Comparative Oncology. Jun2014, Vol. 12 Issue 2, p138-142. 5p.
- Subject
- *GENETIC mutation
*POLYMERASE chain reaction
*MAST cell tumors
*CAT diseases
*GENETIC polymorphisms
*DNA
*FORMALDEHYDE
- Language
- ISSN
- 1476-5810
Here, we describe the establishment of mutant-specific polymerase chain reaction ( PCR) for detection of a c- KIT c. 1430G>T mutation in feline mast cell tumours. Several mutations in feline c- KIT have been identified, with the c. 1430G>T mutation accounting for a significant portion of feline mast cell tumour mutations. The c. 1430G>T mutation in c- KIT exon 9 was detected in 15.7% (11 of 70) of samples by mutant-specific PCR but in only 7.1% (5 of 70) by PCR-restriction fragment length polymorphism ( RFLP) in the genomic DNA isolated from 70 formalin-fixed paraffin-embedded sections or cells collected by fine needle aspiration. Mutant-specific PCR showed remarkably higher detection rate than did PCR-RFLP. DNA sequence analysis did not always yield identical results to those of mutant-specific PCR, suggesting heterogeneity of tumour cells. Mutant-specific PCR is a valid and efficient screening tool for detection of the c- KIT c. 1430G>T point mutation in feline mast cell tumours compared with PCR-RFLP and sequencing analysis. [ABSTRACT FROM AUTHOR]