Selective accumulation of PpIX and photodynamic effect after aminolevulinic acid treatment of human adenomyosis xenografts in nude mice
- Resource Type
- journal article
- Authors
- Suzuki-Kakisaka, Haruka; Murakami, Takashi; Hirano, Toru; Terada, Yukihiro; Yaegashi, Nobuo; Okamura, Kunihiro
- Source
- Fertility & Sterility. Oct2008 Supplement, Vol. 90 Issue 4, p1523-1527. 5p.
- Subject
- *ENDOMETRIOSIS
*THERAPEUTICS
*CLINICAL medicine
*MEDICINE
*MEDICAL care
*REHABILITATION
*AMINO acids
*ANIMALS
*INJECTIONS
*MICE
*PHOTOCHEMOTHERAPY
*PORPHYRINS
*XENOGRAFTS
- Language
- ISSN
- 0015-0282
Objective: To evaluate the effect of photodynamic therapy with aminolevulinic acid (ALA) on human adenomyosis xenografts in a mouse model.Design: Human adenomyosis tissues were implanted SC into nude mice. We measured 5-aminolevulinic acid pharmacokinetics in these mice by analyzing tissue sections 1 to 6 hours after intraperitoneal administration. Twenty-four hours after photodynamic therapy, we evaluated tissue morphologic features.Setting: Department of obstetrics and gynecology at a university hospital in Japan.Patient(s): Immunodeficient mice. Tissue grafts were taken from women with adenomyosis attending a university hospital.Intervention(s): Photodynamic treatment.Main Outcome Measure(s): Peak fluorescence after intraperitoneal ALA administration and tissue histological changes 24 hours after photodynamic therapy.Result(s): Peak fluorescence was observed 3 hours after intraperitoneal administration. Histological studies revealed decreased numbers of epithelial and stromal cells in adenomyosis models after therapy.Conclusion(s): Photodynamic therapy with ALA caused extensive cell death in human adenomyosis tissues implanted into nude mice. Photodynamic treatment using ALA is a potential treatment for patients with adenomyosis uteri. [ABSTRACT FROM AUTHOR]