An investigation was carried out to standardise suitable growing media and growth regulators for enhancing morphological and floral characters of rose at College of Horticulture, Vellanikkara during the period 2018 - 2019. Treatments consisted of three different growing media viz., soil + M-sand + coco peat + FYM, soil + M-sand + cocopeat + vermicompost, soil + M-sand + cocopeat + poultry manure in the ratio of 2:1:1:1 v/v in combination with growth regulators benzyl adenine (BA) and gibberellic acid (GA) at two different levels viz., 200 and 250 ppm. The results revealed that plants grown in growing medium containing FYM with GA at 250 ppm produced superior results with respect to plant height. Vegetative characters such as plant spread, number of branches per plant and number of leaves per branch were higher under the growing media containing FYM along with foliar sprays of BA at 200 and 250 ppm levels. Growing media and growth regulators had significant influence on floral parameters viz., stalk length and number of petals per flower. Media containing FYM (M1) with GA 200 as well as 250 ppm were found to have maximum stalk length. Highest number of petals were observed under media containing FYM (M1) along with BA 200 and 250 ppm, media containing FYM (M1) along with GA 200 and 250 ppm, media containing vermicompost (M2) along with BA 200 ppm and GA 200 ppm as well as media containing poultry manure (M3) along with BA 200 ppm. Early initiation of flower bud was observed under medium containing FYM (M1) along with BA 250 ppm (119.22 days).Growing medium consisting of soil + M-sand + cocopeat + FYM (2:1:1:1) along with monthly application of BA at 200 ppm was found to be effective for enhancing growth and yield of rose under protected condition. [ABSTRACT FROM AUTHOR]