One-pot synthesis of thermosensitive glycopolymers grafted gold nanoparticles and their lectin recognition.
- Resource Type
- Article
- Authors
- Shen, Fa-Wei; Zhou, Kai-Chun; Cai, Hao; Zhang, Yi-Na; Zheng, Yong-Li; Quan, Jing
- Source
- Colloids & Surfaces B: Biointerfaces. Jan2019, Vol. 173, p504-511. 8p.
- Subject
- *GOLD nanoparticles
*LECTINS
*AQUEOUS solutions
*CLUSTERING of particles
*COLLOIDAL stability
- Language
- ISSN
- 0927-7765
Graphical abstract Highlights • Fabrication of thermosensitive glycopolymers grafted gold nanoparticles (Glyco@GNPs) with multi-functionality. • Glyco@GNPs performed remarkable temperature sensitivity and self-assembled in aqueous solution. • Glyco@GNPs showed stronger affinity to lectin Con A, and Con A can induce aggregation of Glyco@GNPs. • The composites of Glyco@GNPs + Con A can inhibit growth of hepatoma cells SMMC-7721. Abstract Thermosensitive glucose-functionalized glycopolymers grafted gold nanoparticles (Glyco@GNPs) with good colloidal stability and thermosensitive in aqueous solution were fabricated by reversible addition-fragmentation chain transfer (RAFT) mediated one-pot synthesis. The formation of core-shell morphology with about a 60 nm gold core in diameter and a glycopolymer shell of about 80 nm in thickness was indicated by transmission electron microscopy (TEM). The recognition ability of the Glyco@GNPs toward lectin concannavalin A (Con A) was verified by ultraviolet-visible spectroscopy and dynamic light scattering (DLS). The good cytocompatibility of the glycopolymers and Glyco@GNPs was proven by MTT assay on L-929 cells. Glyco@GNPs could effectively inhibit hepatoma cells SMMC-7721 growth after recognizing Con A was also proved by MTT assay and flow cytometry assay. [ABSTRACT FROM AUTHOR]