Synthesis and intrinsic optical properties of retinal Schiff base and Green Fluorescent Protein chromophores.
- Resource Type
- Article
- Authors
- Nielsen, Mogens Brøndsted; Petersen, Michael Axman; Andersen, Lars H.
- Source
- AIP Conference Proceedings. 11/14/2008, Vol. 1075 Issue 1, p28-32. 5p. 4 Diagrams, 2 Graphs.
- Subject
- *GREEN fluorescent protein
*FLUORESCENT polymers
*SCHIFF bases
*VISION
*PROTEINS
*MOLECULES
*ABSORPTION
- Language
- ISSN
- 0094-243X
A selection of model systems for the chromophores present in vision proteins (opsins) and the Green Fluorescent Protein (GFP) was synthesized and their intrinsic optical properties were investigated in the gas phase. These investigations have provided fundamental knowledge on protein-chromophore interactions. Thus, opsins induce a blue-shift in the absorption maximum of the retinal Schiff base chromophore, while red- and blue-shifting interactions between GFP and its chromophore cancel out. [ABSTRACT FROM AUTHOR]