Immunoassay based on monoclonal antibodies versus LC-MS: deoxynivalenol in wheat and flour in Southern Brazil.
- Resource Type
- Article
- Authors
- Dos Santos, J.Sifuentes; Takabayashi, C.R.; Ono, E.Y.S.; Itano, E.N.; Mallmann, C.A.; Kawamura, O.; Hirooka, E.Y.
- Source
- Food Additives & Contaminants. Part A: Chemistry, Analysis, Control, Exposure & Risk Assessment. Aug2011, Vol. 28 Issue 8, p1083-1090. 8p.
- Subject
- *ENZYME-linked immunosorbent assay
*MONOCLONAL antibodies
*MYCOTOXINS
*TRICHOTHECENES
*CEREALS as food
*WEIGHT gain
- Language
- ISSN
- 1944-0049
An indirect competitive enzyme-linked immunosorbent assay (ELISA) method using a monoclonal antibody for deoxynivalenol (DON) detection in wheat and flour was standardised and validated (detection limit = 177.1 µg kg−1) and its performance was compared with LC-MS, quantification limit =140 µg kg−1). DON recovery ranged from 88.7% to 122.6% for wheat grain and from 70.6% to 139.3% for flour. Among the 38 wheat samples evaluated, DON was detected in 29 samples (76.3%) by ic-ELISA (281.6–12 291.4 µg kg−1) and in 22 samples (57.9%) by LC-MS (155.3–9906.9 µg kg−1). The 0.93 correlation coefficient between ic-ELISA and LC-MS data in 19 positive DON wheat samples demonstrated the reliability and efficiency of ic-ELISA. Results indicated that standardised ic-ELISA was suitable for DON screening in wheat samples and the need for continuous monitoring of mycotoxin levels in foodstuffs. [ABSTRACT FROM AUTHOR]