Introduction: Left-handedness is a condition whose etiology is unknown. Because cerebral cortex development continues until the 32nd week, it is thought that ultrasound exposure during this period may affect lateralization. The hypothesis we intend to examine in this study is that the frequency of left-handedness increases in children of pregnant women with higher exposure to 2D and Doppler ultrasound than in other groups. Method: Lateralization will be questioned by the Edinburgh Questionnaire to be conducted among pregnant women who were exposed to ultrasound during routine ultrasound examinations and among the children of pregnant women whose ultrasound exposure was higher than others who applied to Bezmialem Vakıf University, Faculty of Medicine, Department of Obstetrics and Gynecology Polyclinic between 2020 and 2023. The questionnaire will be administered online using Google Forms. Results: The total number of participants who met the conditions for participation was 43. While the number of participants who had ultrasonography (USG) exposure for less than 1 hour during the 3rd trimester was 23 (53.48%), according to the inventory, the number of left-handers among these participants was 4 (17.39%), while the number of participants with a total USG exposure of 1 hour or more during the 3rd trimester was observed as 20 (46.42%), and according to the inventory, the number of left-handed participants was 3 (15%). Conclusion: In this retrospective cohort study, depending on the duration of USG exposure, a group with a history of USG exposure of less than 1 hour in the 3rd trimester was compared with a group with a history of ultrasound exposure of 1 hour or more. Prenatal USG exposure did not create a statistically significant difference in left-handedness (p>0.375). Our results need to be confirmed by further survey studies. [ABSTRACT FROM AUTHOR]