Food colors such as tartrazine (E102), ammonia caramel (E150c), sulphite ammonia caramel (E150d) are widely used in the food sector. These additives are thought to be a source of long-term toxicity source. The goal of our research was to emphasize the cytotoxic and genotoxic effects of the three food colors at various concentrations (0.5%, 1 %, and 2%) using the Allium cepa test. The species is thought to be one of the best for assessing genotoxicity because of its low chromosomal number and lengthy chromosomes. The findings revealed that the three dyes have a cytotoxic impact, as seen by root growth inhibition after 120 h of incubation. The three food dyes had a genotoxic effect, as measured by a decrease in the mitotic index and an increase in the frequency of chromosomal aberrations such as chromosomal bridge, stickiness, and vagrant chromosomes, at both concentrations of 0.5% and 1%. At 2%, the mitotic index was reported as 0 and several cytological abnormalities (binucleate and micronucleated cells and fragmented nuclei) were noted. However, further in vitro and in vivo cytogenetic experiments treating cytotoxicity and genotoxicity of the three food dyes using alternative test models (animals, cell lines) will be needed to better understand their mechanisms of action. [ABSTRACT FROM AUTHOR]