Highlights • All pasture meters accurately estimate pasture mass. • Laser sensor was more accurate than other pasture meters estimating pasture mass. • Pasture density measured with the laser improved accuracy of pasture mass estimates. Abstract Pasture meters are typically calibrated to estimate total pasture mass (TPM). Recent studies have shown that animal productivity was driven by the level of utilisation of the top leafy stratum (TLS) rather than the TPM. A study of kikuyu (Pennisetum clandestinum) pasture conducted on two farms located in southeast Queensland, Australia assessed whether pasture mass of the TLS, bottom stemmy stratum (BSS) and TPM can be more accurately estimated by using not only pasture surface height but also pasture density derived from height data captured by a laser sensor. The results indicated that there was a notable improvement of the pasture mass estimates when density was included in the calibration equations. The study also compared the accuracy of the pasture mass estimates between the laser sensor and mechanical pasture meters (rising plate meter and ruler). The results indicated that all devices provided reasonable estimates of pasture mass for TLS, BSS and total (R2 ≥ 0.75). However, pasture mass was estimated with a slightly higher level of accuracy when using the laser sensor in comparison with the other pasture meters, particularly for the top leafy stratum (R2 = 0.81 vs R2 ≤ 0.77). [ABSTRACT FROM AUTHOR]