Isolation of human monocytes by rosetting with antibody coated human erythrocytes and isopycnic gradient centrifugation.
- Resource Type
- Article
- Authors
- Zembala, M.; Uracz, W.; Ruggiero, Irena; Lemmel, E. M.
- Source
- Clinical & Experimental Immunology. Oct1982, Vol. 50 Issue 1, p225-229. 5p.
- Subject
- *ERYTHROCYTES
*MONOCYTES
*MITOGENS
*IMMUNOGLOBULINS
*LEUCOCYTES
*CELL culture
*BLOOD cells
- Language
- ISSN
- 0009-9104
Human erythrocytes O,Rh+(D) coated with hyperimmune human anti-serum formed rosettes primarily with monocytes in peripheral blood mononuclear cell suspensions. The rosetting cells (EAhu-FRC). separated on a Lymphoprep gradient were found in the pellet and were up to 95% pure monocytes as judged by cytochemical criteria. A two step procedure involving depletion of T lymphocytes followed by the isolation of EAhu-RFC yielded monocytes up to 98% pure. The isolated cells did not respond to mitogens in vitro, but they act as accessory cells in mitogen-induced T lymphocyte proliferation. This simple method may be useful in studying monocyte function in human disease as it does not require a large amount of blood. [ABSTRACT FROM AUTHOR]