Electrochemical detection of asparaginase antibodies using bifunctionalized carbon interfaces.
- Resource Type
- Article
- Authors
- Marshall, Daisy; Lehr, Joshua
- Source
- Electroanalysis. Jul2023, Vol. 35 Issue 7, p1-6. 6p.
- Subject
- *ASPARAGINASE
*IMMUNOGLOBULINS
*DETECTION limit
*CARBON
*GENETIC transduction
- Language
- ISSN
- 1040-0397
The early detection of anti‐asparaginase biomarker can facilitate timely modification of asparaginase chemotherapy, thereby avoiding serious complications. Herein we describe the preparation of a novel electrochemical biosensing interface for rapid detection of anti‐asparaginase in the picomolar range (1–10 000 pM). Coimmobilization of ferrocene and asparaginase on a carbon interface (via diazonium grafting) facilitates transduction through attenuation of the surface‐bound ferrocene redox couple. The limit of detection of 0.8 pM for this point‐of‐care applicable method compares favourably to that of traditional faradaic assaying (2.0 pM) where transduction occurs by the target blocking the diffusion of the solution redox probe [Fe(CN)6]3−/4−. [ABSTRACT FROM AUTHOR]