Plants can be used to produce recombinant functional proteins such as antibodies and vaccines. In this study, transgenic plants were utilized to express anti-human programmed death ligand-1 (PD-L1) monoclonal antibodies (mAbP PD-L1) tagged with the endoplasmic reticulum retention motif KDEL. PCR and immunoblot analyses confirmed that mAb PD-L1 was expressed in transgenic plants. ELISA analysis confirmed the binding activity of mAbP PD-L1 to human prostate and urothelial cancer cell lines PC-3 and TCCSUP, respectively. Confocal imaging analysis demonstrated that mAbP PD-L1 had binding activity against human cancer cell lines similar to Avelumab (mAbM PD-L1). Confocal imaging analysis showed that the binding activity of mAbP PD-L1 to the human tonsil is comparable to mAbM PD-L1. These results suggest that mAbP PD-L1 shows functional binding activities for PD-L1+ cancer cells. Hence, this study showed that tobacco plants can be used to produce functional anti-human PD-L1 mAbs. [ABSTRACT FROM AUTHOR]