Intraocular dendritic cells characterize HLA-B27-associated acute anterior uveitis.
- Resource Type
- Article
- Authors
- Kasper, Maren; Heming, Michael; Schafflick, David; Xiaolin Li; Lautwein, Tobias; zu Horste, Melissa Meyer; Bauer, Dirk; Walscheid, Karoline; Wiendl, Heinz; Loser, Karin; Heiligenhaus, Arnd; zu Hörste, Gerd Meyer
- Source
- eLife. 11/16/2021, p1-20. 20p.
- Subject
- *IRIDOCYCLITIS
*MYELOID cells
*EYE diseases
*PROTEIN analysis
*HLA-B27 antigen
- Language
- ISSN
- 2050-084X
Uveitis describes a heterogeneous group of inflammatory eye diseases characterized by infiltration of leukocytes into the uveal tissues. Uveitis associated with the HLA haplotype B27 (HLA-B27) is a common subtype of uveitis and a prototypical ocular immune-mediated disease. Local immune mechanisms driving human uveitis are poorly characterized mainly due to the limited available biomaterial and subsequent technical limitations. Here, we provide the first high-resolution characterization of intraocular leukocytes in HLA-B27-positive (n = 4) and -negative (n = 2) anterior uveitis and an infectious endophthalmitis control (n = 1) by combining single-cell RNA-sequencing with flow cytometry and protein analysis. Ocular cell infiltrates consisted primarily of lymphocytes in both subtypes of uveitis and of myeloid cells in infectious endophthalmitis. HLA-B27-positive uveitis exclusively featured a plasmacytoid and classical dendritic cell (cDC) infiltrate. Moreover, cDCs were central in predicted local cell-cell communication. This suggests a unique pattern of ocular leukocyte infiltration in HLA-B27-positive uveitis with relevance to DCs. [ABSTRACT FROM AUTHOR]