INCREASE IN SUBCELLULAR GSK-3 CLUSTERS IN INSULIN- AND ADRENALINE-TREATED DIFFERENTIATED RAT SKELETAL MUSCLE FIBRES.
- Resource Type
- Article
- Authors
- FINK, KATJA; PREBIL, MATEJA LOBE; VARDJAN, NINA; JENSEN, JØRGEN; ZOREC, ROBERT; KREFT, MARKO
- Source
- Image Analysis & Stereology. 2020, Vol. 39 Issue 1, p25-32. 8p.
- Subject
- *SKELETAL muscle
*GLYCOGEN synthase kinase
*FIBERS
*METABOLIC regulation
*CONFOCAL microscopy
- Language
- ISSN
- 1580-3139
Glycogen synthase kinase 3 (GSK-3) plays an important role in metabolic regulation in skeletal muscles, and both insulin and adrenaline stimulate GSK-3 phosphorylation. The aim of the present study was to study the effect of insulin and adrenaline on GSK-3 localisation in skeletal muscles. We characterized subcellular localization of GSK-3 signal protein in fully differentiated muscle fibre by immunofluorescence and confocal microscopy. We stimulated muscle fibres with insulin and/or adrenaline. Images were analysed by segmentation of single central optical section of the muscle. We found GSK-3 to be localised in clusters. The number of GSK-3 clusters and their average size were increased after stimulation with insulin and/or adrenaline. Average GSK-3 particle size is linearly related to their quantity. We conclude that subcellular GSK-3 in isolated skeletal muscle fibres is localized in clusters and clustering increased after stimulation with insulin and/or adrenaline. [ABSTRACT FROM AUTHOR]