Teaching old indicators even more tricks: binding affinity measurements with the guest-displacement assay (GDA).
- Resource Type
- Article
- Authors
- Sinn, Stephan; Krämer, Joana; Biedermann, Frank
- Source
- Chemical Communications. 6/21/2020, Vol. 56 Issue 49, p6620-6623. 4p.
- Subject
- *MEASUREMENT
*HOSPITALITY
- Language
- ISSN
- 1359-7345
A simple change has important consequences: the guest-displacement assay (GDA) is introduced which allows for binding affinity determinations of supramolecular complexes with spectroscopically silent hosts and guests. GDA is complementary to indicator-displacement assay for affinity measurements with soluble components, but is superior for insoluble or for weakly binding guests. [ABSTRACT FROM AUTHOR]