Nanophotonic immunoarray with electrochemically roughened surfaces for handheld detection of secreted PD-L1 to predict immuno-oncology efficacy.
- Resource Type
- Article
- Authors
- Dey, Shuvashis; Koo, Kevin M.; Ahmed, Emtiaz; Trau, Matt
- Source
- Lab on a Chip. 8/7/2023, Vol. 23 Issue 15, p3443-3452. 10p.
- Subject
- *SERS spectroscopy
*IMMUNE checkpoint proteins
*PROGRAMMED death-ligand 1
*ENZYME-linked immunosorbent assay
*PROTEIN analysis
*PROGRAMMED cell death 1 receptors
*IMMUNOCHEMISTRY
- Language
- ISSN
- 1473-0197
The analysis of secreted protein biomarkers can be a useful non-invasive method of predicting or monitoring cancer therapeutic response. The increased level of soluble programmed cell death protein ligand 1 (sPD-L1) is a promising predictive biomarker for selecting patients who are likely to respond to immune checkpoint immunotherapy. The current established immunoassay for secreted protein analysis is enzyme-linked immunosorbent assay (ELISA). Yet, ELISA is generally still liable to limited detection sensitivity and restricted to bulky chromogenic readout equipment. Herein, we present a designed nanophotonic immunoarray sensor which achieved sPD-L1 analysis at high-throughput, enhanced detection sensitivity and portability. The key benefits of our nanophotonic immunoarray sensor are (i) high-throughput surface-enhanced Raman scattering (SERS) analysis of multiple samples on a singular platform; (ii) improved sPD-L1 detection sensitivity at 1 pg mL−1 (by two orders of magnitude as compared to ELISA) via electrochemically roughened gold sensor surfaces; (iii) fit for handheld SERS detection with miniaturized equipment footprint. We evaluated the analytical performance of the nanophotonic immunoarray sensor and successfully demonstrated quantitative sPD-L1 detection in a cohort of contrived human plasma samples. [ABSTRACT FROM AUTHOR]