A CMP-N-acetylneuraminic Acid Synthetase Purified from a Marine Bacterium, Photobacterium leiognathi JT-SHIZ-145.
- Resource Type
- Article
- Authors
- Kajiwara, Hitomi; Mine, Toshiki; Miyazaki, Tatsuo; Yamamoto, Takeshi
- Source
- Bioscience, Biotechnology & Biochemistry. Jan2011, Vol. 75 Issue 1, p47-53. 7p.
- Subject
- *PHOTOBACTERIUM
*LEIOGNATHIDAE
*GEL permeation chromatography
*ANIONS
*CHROMATOGRAPHIC analysis
- Language
- ISSN
- 0916-8451
The article focuses on the crude extract prepared from Photobacterium leiognathi in which a cytidine 5'-monophospho-N-accetylneuraminic acid (CMP-Neu5Ac) synthetase. It mentions that Photobacterium leiognathi is a marine bacterium which creates a ß-galactoside α2,6-sialyltransferase . It notes that CMP-Neu5Ac synthetase has been purified from the crude extract of the cells through a combination of gel filtration column chromatography and anion-exchange.