[Objectives] Peptides was extracted from Haemadipsa hainana and its activity was studied. [Methods] Electric stimulation, water extraction and ul-trasonic extraction were used to extract the peptides from H hainana. Then the protein content and molecular weight distribution of H haipana peptides were de-tected by the BCA method and SDS-PAGE method, respectively. The antithrombin activity and analgesic activity of the three peptide extracts of H haipana were detected by Markwardt thrombin titration method and mouse hot plate experiment, respectively. [Results] There extraction methods of electric stimulation, water extraction and ultrasonic extraction were used to extract the peptide extract of H haiiana, and the yields were as foUows: water extraction > electrical stimulation > ultrasonic extraction. The three peptide extracts from H hainana had antithrombin activity, and the antithrombin activity was as foUows: water extraction > ultrasonic extraction > electrical stimulation. Through the hot plate experiment in mice, it was verified that the three peptide extracts of H haiiana had analgesic activity, and the analgesic activity was water extraction > electric stimulation > ultrasonic extraction. The analgesic activity of high-dose (100 mg/kg) group of H hainana obtained by water extraction was slightly weaker than that of tramadol. [ Conclusions] This study confirmed that the peptide extract of H haiiana had certain antithrombin and analgesic activity, laying a foundation for the subsequent development and application of H haiiana. [ABSTRACT FROM AUTHOR]