• Defects in additive manufactured samples are characterized by laser ultrasound. • The detection accuracy can reach 300μm, confirmed by X-ray computed tomography. • Build theoretical model and simulation results are confirmed by the experimental. With the development of 3D printing technique, additive manufactured (AM) components are becoming more and more important in the fields of manufacturing industry. However, in the process of manufacturing and utilizing, it is necessary to inspect the appearance of internal defects to ensure safety and to reduce economic losses. In this paper, a pulsed laser is used to generate the ultrasonic waves inside Nickel (Ni) and Titanium (Ti) based alloy samples with internal defects of different diameters, and the propagating ultrasonic waves are detected by an optical interferometer. By analyzing the experimental results, defects with diameters greater than 300 μm in the Ni based alloy sample and that in the Ti based alloy sample can be detected, and this can be validated by X-ray computed tomography. In addition, the effects of ultrasonic waves propagating inside the samples and interacting with the defects are studied theoretically. The experimental results are consistent with the numerical simulation results. These results present that laser ultrasonic technology can evaluate the reliability of AM components, and has the potential to inspect AM components during their production as a non-contact method. [ABSTRACT FROM AUTHOR]