Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.).
- Resource Type
- Article
- Authors
- Seong, Eun Soo; Yoo, Ji Hye; Choi, Jae Hoo; Kim, Chang Heum; Jeon, Mi Ran; Kang, Byeong Ju; Lee, Jae Geun; Choi, Seon Kang; Ghimire, Bimal Kumar; Yu, Chang Yeon
- Source
- International Journal of Genomics. 11/19/2015, p1-7. 7p. 3 Charts, 3 Graphs.
- Subject
- *EXPRESSED sequence tag (Genetics)
*BIOMARKERS
*ANTISENSE DNA
*GENE libraries
*PERILLA frutescens
*MEDICINAL plants
*COMPARATIVE genomics
- Language
- ISSN
- 2314-436X
Perilla frutescens is valuable as a medicinal plant as well as a natural medicine and functional food. However, comparative genomics analyses of P. frutescens are limited due to a lack of gene annotations and characterization. A full-length cDNA library from P. frutescens leaves was constructed to identify functional gene clusters and probable EST-SSR markers via analysis of 1,056 expressed sequence tags. Unigene assembly was performed using basic local alignment search tool (BLAST) homology searches and annotated Gene Ontology (GO). A total of 18 simple sequence repeats (SSRs) were designed as primer pairs. This study is the first to report comparative genomics and EST-SSR markers from P. frutescens will help gene discovery and provide an important source for functional genomics and molecular genetic research in this interesting medicinal plant. [ABSTRACT FROM AUTHOR]