Introduction: Pregnancy is a very sensitive period that is often accompanied by changes in the couple's sexual relationship due to various physical and psychological changes. Aim: The purpose of this study was to evaluate men's sexuality and their attitude toward sexual relationship during their wive's pregnancy. Materials and Methods: The present cross-sectional study was conducted on the husbands of 272 pregnant women who attended healthcare centers of Jahrom, Iran, from March 2016 to May 2016. International Index of Erectile Function (IIEF) questionnaire was used to assess the sexual function. T-test utilised to compare overall satisfaction score with characteristics of the participants, ANOVA test used in order to compare domains of male sexual function with pregnancy trimesters. Besides, p-value <0.05 was considered as statistically significant. Results: The husband's mean age was 32.57±6.12 years. Of the total 62, 81, and 129 subjects were in their first, second and third trimesters, respectively. Comparison of the first, second and the third trimesters revealed a significant difference in the scores of all IIEF domains. Among the study subjects, 82.7% had erectile dysfunction, 84.2% had orgasmic dysfunction, 92.64% had sexual desire dysfunction, 95.6% had intercourse dissatisfaction, and 80.1% had overall dissatisfaction. Almost 62.2% of the men displayed a negative attitude towards sexuality and only 11% men had positive attitude. Conclusion: The results of the present study showed that sexual disorders were highly prevalent among men. The most prevalent sexual dysfunction was desire disorder and intercourse dissatisfaction. Since, attitude towards sexuality affected their general sexual behaviour during pregnancy, it is crucial to provide proper consultation regarding sexual relations in prenatal care services. [ABSTRACT FROM AUTHOR]