• A non-Fourier prediction methodology of triple-layer cutaneous tissue to evaluate skin burn injury • Increase in thermal conductivity of the epidermis decreases the thermal resistance and results in increased heat penetration of the tissue • Higher thermal conductivity of the tissue lowers the degree of burn injury • Increase in dermis thermal conductivity results in a prolonged time to reach the injury threshold • Blood perfusion rate exhibits negligible net effect on the prediction of burn injury in the dermis layer. • Relaxation time, thermalization time and initial skin tissue temperature exhibits significant effects on the outcome of burn injury exposure • Heat transfer coefficient plays significant effects on burn injury for small exposure time • The most dominating factors in the burn injury follow the order: relaxation time and thermalization time, initial tissue temperature followed by the epidermis layer thermal conductivity, dermis layer thermal conductivity and convective heat transfer coefficient This paper demonstrates a non-Fourier prediction methodology of triple-layer human skin tissue for determining skin burn injury with non-ideal properties of tissue, metabolism and blood perfusion. The dual-phase lag (DPL) bioheat model is employed and solved using joint integral transform (JIT) through Laplace and Fourier transforms methods. Parametric studies on the effects of skin tissue properties, initial temperature, blood perfusion rate and heat transfer parameters for the thermal response and exposure time of the layers of the skin tissue are carried out. The study demonstrates that the initial tissue temperature, the thermal conductivity of the epidermis and dermis, relaxation time, thermalisation time and convective heat transfer coefficient are critical parameters to examine skin burn injury threshold. The study also shows that thermal conductivity and the blood perfusion rate exhibits negligible effects on the burn injury threshold. The objective of the present study is to support the accurate quantification and assessment of skin burn injury for reliable experimentation, design and optimisation of thermal therapy delivery. [ABSTRACT FROM AUTHOR]