In this Article, we present a new strategy for preparingan antihemoglobinbiointerface on cellulose. The preparation method is based on functionalizationof the cellulose surface by the irreversible adsorption of CMC, followedby covalent linking of antibodies to CMC. This would provide the meansfor affordable and stable cellulose-based biointerfaces for immunoassays.The preparation and characterization of the biointerface were studiedon LangmuirâSchaefer cellulose model surfaces in real timeusing the quartz crystal microbalance with dissipation and surfaceplasmon resonance techniques. The stable attachment of antihemoglobinto adsorbed CMC was achieved, and a linear calibration of hemoglobinwas obtained. CMC modification was also observed to prevent nonspecificprotein adsorption. The antihemoglobin-CMC surface regenerated well,enabling repeated immunodetection cycles of hemoglobin on the samesurface. [ABSTRACT FROM AUTHOR]