Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin.
- Resource Type
- Article
- Authors
- Wang, Guan; Kitaoka, Traci; Crawford, Ali; Mao, Qian; Hesketh, Andrew; Guppy, Fergus M.; Ash, Garrett I.; Liu, Jason; Gerstein, Mark B.; Pitsiladis, Yannis P.
- Source
- Scientific Reports. 11/4/2021, Vol. 11 Issue 1, p1-14. 14p.
- Subject
- *RECOMBINANT erythropoietin
*TRANSCRIPTOMES
*GENE expression
*KNOWLEDGE base
*BIOLOGY
- Language
- ISSN
- 2045-2322
RNA-seq has matured and become an important tool for studying RNA biology. Here we compared two RNA-seq (MGI DNBSEQ and Illumina NextSeq 500) and two microarray platforms (GeneChip Human Transcriptome Array 2.0 and Illumina Expression BeadChip) in healthy individuals administered recombinant human erythropoietin for transcriptome-wide quantification of differential gene expression. The results show that total RNA DNB-seq generated a multitude of target genes compared to other platforms. Pathway enrichment analyses revealed genes correlate to not only erythropoiesis and oxygen transport but also a wide range of other functions, such as tissue protection and immune regulation. This study provides a knowledge base of genes relevant to EPO biology through cross-platform comparisons and validation. [ABSTRACT FROM AUTHOR]