Identification of an atypical calcium-dependent calmodulin binding site on the C-terminal domain of GluN2A.
- Resource Type
- Article
- Authors
- Bajaj, Gaurav; Hau, Andrew M.; Hsu, Peter; Gafken, Philip R.; Schimerlik, Michael I.; Ishmael, Jane E.
- Source
- Biochemical & Biophysical Research Communications. Feb2014, Vol. 444 Issue 4, p588-594. 7p.
- Subject
- *CALMODULIN
*BINDING sites
*C-terminal binding proteins
*CALCIUM ions
*BIOCHEMISTRY
*MASS spectrometry
- Language
- ISSN
- 0006-291X
Highlights: [•] Calmodulin was identified by MS as a GluN2A C-terminus-associated protein. [•] Ca2 +/calmodulin bound directly to GluN2A with high affinity (5.2±2.4nM) in vitro. [•] Disruption of classic Ca2+/calmodulin recognition motifs did not affect binding. [•] Trp-1014 of GluN2A was a critical determinant of Ca2+/calmodulin binding in vitro. [ABSTRACT FROM AUTHOR]