Detection and quantification of HIV-1 specific CD4 helper and CD8 cytotoxic cells: their role in HIV-1-infected individuals and vaccine recipients.
- Resource Type
- Article
- Authors
- Imami, N; Hardy, G; Pires, A; Burton, C; Sullivan, A; Gotch, F
- Source
- HIV Medicine. Jul2001, Vol. 2 Issue 3, p146-153. 8p.
- Subject
- *HIV infections
*T cells
*CD4 antigen
- Language
- ISSN
- 1464-2662
There is a strong link between virus specific CD8 T-cell function and the efficiency of regulatory CD4 helper T cells. Controlling viraemia in HIV-1-infected individuals requires the maintenance of strong CD4 and CD8 T-cell responses. These responses should be elicited by prophylactic vaccination and by postexposure immunotherapy. This review will examine the methods that are available for the detection and quantification of HIV-1 specific CD4 and CD8 T-cell responses. We will also discuss the methods that should be used to identify these responses in HIV-1-infected individuals, seropositive recipients of immunotherapy and seronegative vaccinees. Finally, we will give examples of how responses observed in vitro relate to those known to occur in vivo. [ABSTRACT FROM AUTHOR]