Facile Synthesis of Fluorescent Latex Nanoparticleswith Selective Binding Properties Using Amphiphilic Glycosylated PolypeptideSurfactants.
- Resource Type
- Article
- Authors
- Jacobs, J.; Byrne, A.; Gathergood, N.; Keyes, T. E.; Heuts, J. P.A.; Heise, A.
- Source
- Macromolecules. Nov2014, Vol. 47 Issue 21, p7303-7310. 8p.
- Subject
- *NANOPARTICLE synthesis
*LATEX
*SURFACE active agents
*FLUORESCENCE
*POLYPEPTIDES
*RUBBER
*AMPHIPHILES
*BLOCK copolymers
- Language
- ISSN
- 0024-9297
Block copolymers comprising a poly(styrene)and a poly(l-lysine) or poly(l-glutamic acid) blockwere obtained bysequential reversible addition–fragmentation chain Transfer(RAFT) and N-carboxyanhydride (NCA) polymerization.Subsequent partial glycosylation of the poly(l-glutamic acid)block with d-galactosamine (GA) and the poly(l-lysine)block with lactobionic acid (LA) yielded block copolymers with a degreeof glycosylation of 50% and 35%, respectively, in the poly(amino acid)block. These amphiphilic block copolymers were successfully employedas macromolecular surfactants in the emulsion polymerization of styreneto produce uniform 100–150 nm size nanoparticles with a poly(syrene)core and galactose containing poly(l-amino acid) periphery.Introduction of fluorescence was achieved by incorporation of NileRed during latex formation and reaction of remaining lysine functionalitieson the nanoparticle periphery with fluorescein isothiocyanate (FITC).The availability of the galactose units at the nanoparticles surfacefor selective binding was demonstrated by lectin binding experimentsand binding to Chinese hamster ovary (CHO) cells. Confocal imagesof live CHO cells following incubation with fluorescent glycosylatednanoparticles confirmed that the nanoparticles bound strongly to thecell surface and could only be removed by addition of free lactobionicacid, highlighting selective binding of the nanoparticles on the cellsurface. [ABSTRACT FROM AUTHOR]