Detection of heavy metal ions in water is important for environmental sustainability and food safety. Current fluorescent sensors interact with metal ions directly through chelation or chemical reactions. Those sensors are expensive to produce and often can detect only one ion at a time. Here we report a fluorescent turn-on sensor that can detect three group IIB metal ions and Pb 2+ ions through label-free polypeptides in water. In our sensor-polypeptide mixture, Zn 2+ , Cd 2+ , Hg 2+ , and Pb 2+ ions induce helix formation and inter-chain aggregation in poly- L -α-glutamic acid (PGA). The acridinium-based sensor molecules incorporate into the polypeptides and emit strongly with characteristic color for each group IIB ion under UV lamp. By adjusting the size of polypeptides or the length of the side chain carboxyl groups, we can selectively turn off or turn on the sensor emission for Hg 2+ ions. [ABSTRACT FROM AUTHOR]