Four new megastigmane glycosides, eucomegastigsides A-D ( 2 , 3 , 5 and 7 ), together with three known megastigmane glycosides, (6 R , 7 E , 9 R )-9-hydroxy-4, 7-megastigmadien-3-one-9- O -[ α - l -arabinopyranosyl-(l → 6)- β - d -glucopyranoside ( 1 ), foliasalacioside B 1 ( 4 ) and eleganoside A ( 6 ), were isolated from the leaves of Eucommia ulmoides Oliver. Their anti-hypertensive effect was investigated in vitro based on the inhibition of Angiotensin Converting Enzyme (ACE) using HPLC. The results showed that the isolates ( 2 , 3 , 4 , 5 , 7 ) had moderate inhibitory effects on ACE in vitro compared with captopril. [ABSTRACT FROM AUTHOR]