Molecular characterization of a glycosyl hydrolase family 10 xylanase from Aspergillus niger.
- Resource Type
- Article
- Authors
- Do, Thi Tuyen; Quyen, Dinh Thi; Nguyen, Thi Nuong; Nguyen, Van Thuat
- Source
- Protein Expression & Purification. Dec2013, Vol. 92 Issue 2, p196-202. 7p.
- Subject
- *HYDROLASES
*XYLANASES
*ASPERGILLUS niger
*MOLECULAR biology
*FUNGAL gene expression
*RECOMBINANT proteins
*ACETONE
- Language
- ISSN
- 1046-5928
Highlights: [•] First report about cloning and expression of a glycosyl hydrolase family 10 xylanase from Aspergillus niger in P. pastoris. [•] A thorough study on physicochemical properties of the recombinant GH family 10 xylanase from A. niger. [•] Stable at a broad pH range of 4.5–8.5 and resistant to detergents and acetone: potential for enzymatic reactions. [•] Hydrolysis endproducts of birch wood xylan were xylotriose, xylotetraose, and xylopentose. [Copyright &y& Elsevier]