Characterization of the Temperature-Sensitive Mutations un-7 and png-1 in Neurospora crassa.
- Resource Type
- Article
- Authors
- Dieterle, Michael G.; Wiest, Aric E.; Plamann, Mike; McCluskey, Kevin
- Source
- PLoS ONE. 2010, Vol. 5 Issue 5, p1-5. 5p. 1 Black and White Photograph, 2 Diagrams, 4 Charts.
- Subject
- *GENETIC mutation
*NEUROSPORA crassa
*MORPHOLOGY
*PHENOTYPES
*PHENYLALANINE
*SERINE
*LEUCINE
- Language
- ISSN
- 1932-6203
The model filamentous fungus Neurospora crassa has been studied for over fifty years and many temperature-sensitive mutants have been generated. While most of these have been mapped genetically, many remain anonymous. The mutation in the N. crassa temperature-sensitive lethal mutant un-7 was identified by a complementation based approach as being in the open reading frame designated NCU00651 on linkage group I. Other mutations in this gene have been identified that lead to a temperature-sensitive morphological phenotype called png-1. The mutations underlying un-7 result in a serine to phenylalanine change at position 273 and an isoleucine to valine change at position 390, while the mutation in png-1 was found to result in a serine to leucine change at position 279 although there were other conservative changes in this allele. The overall morphology of the strain carrying the un-7 mutation is compared to strains carrying the png-1 mutation and these mutations are evaluated in the context of other temperature-sensitive mutants in Neurospora. [ABSTRACT FROM AUTHOR]